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This Concept Map, created with IHMC CmapTools, has information related to: LAB 1- INTRODUCTION TO THE MICROSCOPE AND COMPARISON OF SIZES AND SHAPES OF MICROORGANISMS, coccus: spherical or oval arrangements sarcina: cube of 8 cocci; division in three planes, spiral forms vibrio: comma-shaped, coccus: spherical or oval arrangements streptococcus: cocci in chains; division in one plane, coccus: spherical or oval arrangements diplococcus: cocci in pairs; division in one plane, spiral forms spirochete: thin, flexible spiral, Bacteria are unicellular prokaryotic microorganisms that divide by binary fission. microscopic appearance common bacterial shapes, Focusing with oil immersion microscopy. (1000X) step 1 Before you plug in the microscope, turn the voltage control dial on the right-hand side of the base of the microscope to 1). Now plug in the microscope and turn it on., common bacterial shapes shape name spiral, bacillus (rod): hot dog-shaped arrangements bacillus: single bacillus, coccus: spherical or oval arrangements tetrad: square of 4 cocci; division in two planes, coccus: spherical or oval size typically 0.5-1.0 µm in diameter, coccus: spherical or oval arrangements staphylococcus: cocci in irregular, grape-like clusters; division in random planes, While looking through the eyepieces, turn the fine focus (smaller knob) towards you at a slow steady speed until the specimen comes into focus. step 7 Using the iris diaphragm lever, adjust the light to obtain optimum contrast, Make sure the iris diaphragm lever in front under the stage is almost wide open, (toward the left side of the stage.) step 5 Watching the slide and objective lens carefully from the front of the microscope, lower the oil immersion objective into the oil by raising the stage until the lens just touches the slide. Do this by turning the coarse focus (larger knob) away from you until the spring-loaded objective lens just begins to spring upward., Rotate the white-striped 100X oil immersion objective until it is locked into place. This will give a total magnification of 1000X. step 4 Make sure the iris diaphragm lever in front under the stage is almost wide open, (toward the left side of the stage.), spiral forms spirillum: thick, rigid spiral, bacillus (rod): hot dog-shaped size typically 0.5-1.0 µm wide and 1- 4 µm long, common bacterial shapes shape name bacillus (rod): hot dog-shaped, Before you plug in the microscope, turn the voltage control dial on the right-hand side of the base of the microscope to 1). Now plug in the microscope and turn it on. step 2 Place the slide in the slide holder and place a rounded drop of immersion oil on the area to be observed., spiral size typically 5-100 µm long
